Datasets, processing and refinement details for Mtb-AnPRT: inhibitor structures with various space groups

نویسندگان

  • Genevieve L. Evans
  • Daniel P. Furkert
  • Nacim Abermil
  • Preeti Kundu
  • Katrina M. de Lange
  • Emily J. Parker
  • Margaret A. Brimble
  • Edward N. Baker
  • J. Shaun Lott
چکیده

There are twenty-five published structures of Mycobacterium tuberculosis anthranilate phosphoribosyltransferase (Mtb-AnPRT) that use the same crystallization protocol. The structures include protein complexed with natural and alternative substrates, protein:inhibitor complexes, and variants with mutations of substrate-binding residues. Amongst these are varying space groups (i.e. P21, C2, P21212, P212121). This article outlines experimental details for 3 additional Mtb-AnPRT:inhibitor structures. For one protein:inhibitor complex, two datasets are presented - one generated by crystallization of protein in the presence of the inhibitor and another where a protein crystal was soaked with the inhibitor. Automatic and manual processing of these datasets indicated the same space group for both datasets and thus indicate that the space group differences between structures of Mtb-AnPRT:ligand complexes are not related to the method used to introduce the ligand.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Iterative feature refinement for accurate undersampled MR image reconstruction.

Accelerating MR scan is of great significance for clinical, research and advanced applications, and one main effort to achieve this is the utilization of compressed sensing (CS) theory. Nevertheless, the existing CSMRI approaches still have limitations such as fine structure loss or high computational complexity. This paper proposes a novel iterative feature refinement (IFR) module for accurate...

متن کامل

SInCRe—structural interactome computational resource for Mycobacterium tuberculosis

We have developed an integrated database for Mycobacterium tuberculosis H37Rv (Mtb) that collates information on protein sequences, domain assignments, functional annotation and 3D structural information along with protein-protein and protein-small molecule interactions. SInCRe (Structural Interactome Computational Resource) is developed out of CamBan (Cambridge and Bangalore) collaboration. Th...

متن کامل

Acquisition of cleft structures in L1 and L2

The  present study aims at exploring the processing difficulty of cleft structures as a type of relative clause for EFL and Persian as  first language learners.The impact of head nouns with various functions as well as that of embedding on the processing of Persian and English cleft structures has been investigated in the present study.The participants  were 68  Iranian male and female students...

متن کامل

Morphological and Crystallographic Characterization of Nanoparticles by Granulometry Image Analysis and Rietveld Refinement Methods

The particle size distribution of the resultant cobalt ferrite samples was determined from Scanning Electron Microscopy (SEM) images using the granulometry image analysis method. Results showed the nanosized particles of the samples. The X-Ray Diffraction (XRD) patterns of samples were also analyzed by Rietveld refinement method. The results indicated that the precipitated sample at 95 <sup...

متن کامل

MINING FUZZY TEMPORAL ITEMSETS WITHIN VARIOUS TIME INTERVALS IN QUANTITATIVE DATASETS

This research aims at proposing a new method for discovering frequent temporal itemsets in continuous subsets of a dataset with quantitative transactions. It is important to note that although these temporal itemsets may have relatively high textit{support} or occurrence within particular time intervals, they do not necessarily get similar textit{support} across the whole dataset, which makes i...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره 15  شماره 

صفحات  -

تاریخ انتشار 2017